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The aim of current study was to explore chemical composition and in-vitro antioxidant and antibacterial properties of Pterospermum acerifolium. Very few and sporadic works are there in the literature regarding the phytochemistry and biological activities of P. acerifolium (seed). Qualitative phytochemical analysis reflects the presence of tannins, steroids and flavanoids in the n-hexane extract and ethyl acetate extract of the seed was rich in alkaloids, glycosides, steriods and flavonoids. The n-hexane extract were assessed for antioxidant potential using DPPH free radical scavenging assay. The results showed promising anti-radical activity at 100µg/ml concentration. Evaluation of antibacterial effects was done against Gram-positive and Gram-negative bacteria. The antibacterial activity of P. acerifolium seed extract was measured through determining zone of inhibition by well diffusion method against E. coli and S. aureus. Ethyl acetate seed extract exhibited good antibacterial effects. However, that is comparatively lower as compared to standard (ofloxacin).
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