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Pyrazolo [3,4-d]pyrimidin-4-amine are found most active molecules are synthesized by propanedinitrile, diethoxymethoxyethane and acetic anhydride and boiled at 180 degrees & yielded good products Ethoxy methylidene propanedinitrile which was then boiled and reacted with phenyl hydrazine and yielded 5-amino-1-phenyl-4,5-dihydro-1H-pyrazole-4-carbonitrile and good percentile which then reacted with concentrated sulphuric acid and yielded 5-amino-1-phenyl-4,5-dihydro-1H-pyrazole-4-carboxamide and cyclysed with ester methyl chloro acetate and polymerised the pyrimidine ring the keto functional group on the molecules further chlorinated with phosphorous oxy chloride and yielded with good results 6-(chloromethyl)-1-phenyl-1,5-dihydro-4H-pyrazolo[3,4 -d]pyrimidin-4-one which further reacted with amines are substituted chlorines with amines groups. The antioxidant activity of the molecules was determined by free radical scavenging assay by in vitro method called DPPH assay. All the compounds (A1-A10) were found active in SOD assay procedure. In vivo anti oxidant activity evaluation of antioxidant activity using azathioprine induced oxidative stress in rats, superoxide dismutase: Superoxide dismutase is class of enzyme that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. It is an important antioxidant defence in nearly all cells exposed to oxygen. Superoxide dismutase activity was estimated in tissue homogenate with help of pure bovine superoxide dismutase standard and compounds A1was highly effective with anti oxidant activity.
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