AFFORDABLE REVERSE PHASE FLUID CHROMATOGRAPHIC STRATEGY FOR THE ASSESSMENT OF FOSAMPRENAVIR IN TABLETS

Abstract

A straightforward, exact, fast and affordable reverse phase fluid chromatographic strategy has been produced for the assessment of fosamprenavir in mass and in tablets, utilizing versatile stage containing 20mM Ammonium acetate buffer (pH-3.0) and acetonitrile [50:50 %v/v] at a stream pace of 1.0ml/min. An Phenomenex Luna C18 (250 x 4.6mm i.d, 5µ) column was utilized as a stationary phase. Quantitation was performed utilizing diode exhibit identifier at 225nm and the run time noticed was 4.0min. The retention time of the medication was discovered to be 1.598min. The linearity was seen in the scope of 5.0-15μg/ml with connection coefficient 0.9974. The rate measure of fosamprenavir was 99.5 to 101.2%.The created technique was approved to decide its exactness and accuracy via completing recuperation considers and intra-day and between day examines. Consequently, the current strategy is basic, exact, precise and fast for the assessment of fosamprenavir in tablet measurement structure.